AINIA / Laboratories / Microbiology / Whole genome sequencing (WGS)

Whole genome sequencing (WGS) of bacterial isolates

Advanced genomic characterisation of bacterial isolates for research and microbiological risk management

Genomic characterisationto understand and control microbiological risk

Conventional identification of microorganisms using phenotypic or partial genetic techniques provides, in many cases, insufficient information to address complex situations such as outbreak investigations, contamination traceability, microbial persistence in facilities or advanced microbiological risk assessment.

In a context of increasing demands from authorities, customers and self-monitoring systems, companies need tools that enablein-depth and precise characterisation of bacterial isolates beyond species-level identification.

Having complete genomic information is essential to understand relationships between isolates, compare strains and robustly support decision-making in food safety and hygiene control.

Our solution

Whole genome sequencing of bacterial isolates

The complete sequencing service for bacterial isolates (Whole Genome Sequencing, WGS) offered by AINIA is based on the analysis of the complete genome of bacteria previously isolated using culture techniques.

Genomic characterisation and bioinformatic analysis

Sequencing and the associated bioinformatic analysis enable comprehensive characterisation of the isolate, including its genetic identity, phylogenetic relationships and the presence of genetic elements of interest.

Expert interpretation of results

Expert interpretation of the results is carried out taking into account the context of the product, process and environment, providing highly valuable technical information for traceability studies, incident investigations and support for microbiological risk management.

Applications

Impact and benefits

High resolution in the characterisation and differentiation of bacterial isolates.
Improved traceability and attribution of contamination sources.
Strengthening of the scientific basis for decision-making in complex situations.
Advanced complement to classical microbiology and molecular biology techniques.
Robust technical support in audits, incident investigations or interactions with authorities.

Use case

Traceability of contamination and persistence of strains in the plant

Food companies use complete sequencing of bacterial isolates to investigate the relationship between isolates obtained from products and from surfaces in the plant (traceability), in order to determine possible sources of contamination and assess the persistence of certain strains. Genomic analysis enables precise comparisons between isolates and provides robust technical evidence to guide control and prevention measures.

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Sonia Marco

Head of Microbiology Laboratory

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