Hygiene indicators

Analysis of hygiene indicator microorganisms

Control of hygiene indicators in food production

Monitor hygienic conditions in the production of food and other products through the analysis of indicator microorganisms. The microbiological quality of products affects not only their shelf life, but also their safety as potential vehicles of foodborne illness.

Regulation (EU) No 2073/2005 establishes the microbiological criteria applicable to food products, considering both food safety criteria and process hygiene criteria. These criteria are based on the microbiological analysis of parameters associated with both the presence of pathogens and indicators of poor hygiene, setting levels that are interpreted as satisfactory, acceptable or unsatisfactory depending on the results obtained. Therefore, the analysis of hygiene indicators is a legal requirement for food business operators.

Through the analysis of hygiene indicators, we can:

  • Assess the overall hygienic quality of our products
  • Evaluate the hygienic conditions of equipment, production environments and handling practices
  • Validate and verify cleaning and disinfection processes

Therefore, monitoring hygiene indicators allows us to adopt a genuinely preventive approach, as they enable us to identify inadequate hygiene conditions or practices that increase the risk of contamination by both spoilage microorganisms and pathogens. Indicator monitoring therefore complements the control of pathogens and spoilage microorganisms.

At AINIA, we add value

  • Mesophilic aerobic bacteria
  • Total coliforms
  • Escherichia coli
  • Moulds and yeasts
  • Coagulase-positive staphylococci
  • Enterobacteriaceae
  • Sulfite-reducing anaerobes
  • Spores of sulfite-reducing anaerobes

Plate count, automated MPN, manual MPN, culture-based isolation techniques for detection

Accredited under the “Microbiological Testing of Food” Accreditation Programme (NT-70.02)
Testing for compliance with the microbiological criteria applicable to foodstuffs (Listeria monocytogenes, Salmonella spp., Escherichia coli, aerobic colony count, Enterobacteriaceae, coagulase-positive staphylococci, presumptive Bacillus cereus, staphylococcal enterotoxins).


View accreditation at: ENAC accreditations for AINIA laboratories

Hygiene indicators

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Sonia Marco
Head of Microbiology Laboratory

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